Impact of Maternal and Placental Pathology on Successful Umbilical Cord Blood Sampling and Cryopreservation

J. Marin, M. Calomfirescu, R. Bohâlţea, C. I. Târgovişte

Abstract: Umbilical cord blood (UCB) is an alternative for stem cell transplantation in the treatment of various diseases. In order to find out whether the maternal, placental or fetal pathology before storage might have an impact on the quality of umbilical cord stem cells (UCBSC) and on the success of sampling and cryopreservation, viability and quantity of UCB were assessed. All 145 CB samplings with Biogenis Cord Blood Bank between 2007-2009 of 93 healthy pregnant women and 52 women with pregnancy pathology were investigated in a 3 years retrospective multicenter clinical study. Were analyzed: gestational age at delivery, birth weight, child sex, mode of delivery, pregnancy pathology, umbilical cord and placental pathology. Primary outcomes included umbilical cord blood (UCB) amount, white blood cells (WBC) quantity and viability. Neither gestational age, mode of delivery, child sex, maternal, placental or umbilical pathology affected the WBC quantity. By contrast, cumulative associated maternal pathology negatively affected WBC vitality (p=0.038). The placental pathology significantly influenced both UCB amount and CD34+ concentration. The placental pathology and birthweight are independentely correlated to UCB mass. The unsuccessful collection of UCB was signifficantly correlated with placental pathology (p<0.0001). Data of this study show that perinatal factors are influencing the quality or quantity of UCB. Standard multiple regression models may prove useful for predicting successful UCB mass sampling. In addition, sampling conditions procedures are requisite for pursuing the banking and release of quality UCB for successful transplantation.
Keywords: stem cell, umbilical cord blood, placenta, CD 34+ CD 45+, viability