Abstract: Fetal loss evaluation after amniocentesis (intrauterine demise or miscarriage) 4 weeks after procedure or before 24 weeks’ gestation in case of prophylactic protocol of medication and day monitoring associated to amniocentesis. The study was conducted between 2008 and 2010 and included 143 singleton pregnancies, which underwent amniocentesis at 16-19 weeks in 24 hours hospitalization conditions with morphological evaluation
of fetus, cervix and placental insertion. Antibiotics (cefuroxime axetile 2 g/day, orally) were administered 24 hours before starting the procedure and continued 4 days after. Tocolysis (indometacinum and, optionally, progestin), was maintained at least 24 hours after the complete procedure. Our results were compared with the ones suggested by literature. Keywords: amniocentesis, risk, prophylaxis, protocol.